Haemolysis and Plasma Alkaline Phosphatase Activity

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Alkaline phosphatase activity in the plasma of children and adolescents.

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Alkaline phosphatase isoenzymes in plasma in hyperthyroidism.

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Plasma alkaline phosphatase isoenzymes in hepatobiliary disease.

BY CELLULOSE ACETATE OR ACRYLAMIDE GEL ELECTROPHORESIS IT IS POSSIBLE TO SEPARATE THESE ALKALINE PHOSPHATASE ISOENZYMES FROM SERUM: [anode] fast liver, slow liver, placenta/Regan, bone, intestine, bile [cathode]. Heat or chemical inhibition can confirm the differentiation. Normal adult serum always contains slow-liver isoenzyme, and sometimes bone isoenzyme: the latter is always present in seru...

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High pH values have been found necessary for optimum activity of alkaline phosphatase in vitro. The pH values in the living cell structures, on the other hand, are near neutrality: cytoplasm 6.9, nucleus 7.6 (l-3). This definite difference between the pH required for optimum activity and the pH of the living cell indicates that alkaline phosphatase is not optimally active in viva. However, the ...

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ژورنال

عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine

سال: 1988

ISSN: 0004-5632,1758-1001

DOI: 10.1177/000456328802500211